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Emilia was baptized at the church of St. Botolph, Bishopsgate, on January 27, 1569, the daughter of Baptiste Bassano, a Venice-born musician to Queen Elizabeth, and Margaret Johnson, who was possibly the aunt of Court composer Robert Johnson. She also had a sister, Angela, and two brothers who died before reaching adulthood. Her father died when she was 7, leaving Emilia a dowry of 100 pounds.
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In 1611 she published her volume of poetry, Salve Deus Rex Judaeorum when she was 42. She was one of the first women in England to declare herself a poet, and her readers considered her work quite radical in subject and unusual stylistically. This book centers on the title poem, a work of over 200 stanzas which tells the story of Christ's passion satirically and from the viewpoint of the women around Him. The main poem is prefaced by 10 shorter works dedicated to aristocratic women, beginning with the Queen. After the central poem is "Dedication of Cookham," dedicated to the Clifford women. It's one of the earliest "feminist" works.
After her husband's death, she supported herself by running a school, but little else is known of her life between 1619 and 1635. She had rent disputes with her landlord, and sued her husband's brother for money owed her from his estate (the courts ruled in her favor, but he would not pay her immediately, so she brought suit again--twice). She died at the age of 76 and was buried at Clerkenwell.
One enduring legend of Emilia Lanier is that she could have been Shakespeare's "Dark Lady," which was first proposed by A.L. Rowse. This can never be known for certain, of course, but it's as plausible a theory as any other!
Some good sources are:
David Lasocki and Roger Prior, The Bassanos: Venetian Musicans and Instrument Workers in England 1531-1665
The Diary of Simon Forman (hilariously bawdy reading!)
The Poems of Emilia Lanyer
Stephanie Hopkins Hughes, "New Light on the Dark Lady" (Shakespeare Oxford Newsletter, September 22, 2000)
Susanne Woods, Lanyer: A Renaissance Woman Poet
Martin Green, "Emilia Lanier IS The Dark Lady" (English Studies, Vol. 87 No. 5, October 2006)